You may need to change your Windows sound configuration in order to record from speakers.
In the System Tray, right-click on the speaker icon and choose Recording Devices. In the Sound window, right-click anywhere in the device list and select Show Disabled Devices. Now, check the list for devices named "Wave out mix" and "Stereo Mix". If either is marked "Disabled", right-click on it and enable the device, then click OK.
Now run My Screen Recorder Pro and the Audio Tuning Wizard again, and select either "Wave Out Mix" or "Stereo Mix" as your recording device. You should now be able to record sound from speakers
If neither the "Wave Out Mix" nor the "Stereo Mix" device appears in the list, you may need to update the audio drivers for your system.